Apport de l'immunohistochimie dans le diagnostic des lymphomes B agressifs chez les patients infectes ou non par le VIH en Republique democratique du Congo

Objectif. Determiner la contribution potentielle des techniques immunohistochimiques au diagnostic; et dans la prise en charge therapeutique des lymphomes-B agressifs chez des patients infectes ou non par le VIH; en R D Congo; dans le but de vulgarisation de cette approche; complementaire a l'etude morphologique; et indispensable pour le sous-typage de lymphomes; en particulier; de lymphomes-B agressifs. Methodes. Etude transversale et retrospective de 101 blocs de paraffine portant le diagnostic de lymphome et analyses entre 2005 et 2010; par des techniques morphologiques et immunohistochimiques; dans six laboratoires specialises de notre pays. Resultats. Les 81 blocs retenus; etaient tous CD20 positifs et CD3 negatifs; mais le profil etait variable pour d'autres marqueurs etudies. Le lymphome de Burkitt a ete identifie sur 40 blocs; incluant 7 sujets VIH+ (17;5); les lymphomes B diffus a grandes cellules; sur 35; avec 11; chez les VIH+ (31;4).Six lymphomes de forme intermediaire; dont 3; chez des sujets VIH+ (50). Conclusion. Cette etude a permis de classifier les lymphomes-B agressifs dans notre contexte; grace a l'immunohistochimie; justifiant le recours aux anticorps anti-CD20 pour leur traitement

Validation of expedient methods for measuring body composition in Indian adults.

The body composition of 99 men and 89 women from south India was estimated using hydrodensitometry, bioelectrical impedance and skinfold thickness. Comparisons of the hydrodensitometry (reference method) and skinfold methods showed that there were no significant differences between the methods, for estimates of fat free mass (FFM) and per cent fat. The mean difference between the estimates FFM (bias), from skinfold measurement and hydrodensitometry was small for both groups (+0.16 +/- 1.09 kg in men and +0.67 +/- 0.9 kg in women). The same trend was observed in per cent fat estimates (-0.37 +/- 2.04 in men and -1.49 +/- 2.28 in women), showing that the skinfold method can be used as an accurate and expedient method to determine body composition. The bioelectrical impedance method obtained a significantly lower FFM and higher body fat than the reference (hydrodensitometry) method. This could have been due to the use of an inappropriate equation derived from Western population studies. Hence, a new predictive equation, for the measurement of FFM by the bioelectrical impedance method was derived for this population, using the variables of height2/impedance and FFM measured by underwater weighing. The new equation for the bioelectrical impedance method then gave values of body composition which compared well (0.26 +/- 2.32 kg) in men and (0.36 +/- 2.49 kg) in women with the hydrodensitometry method.

Body composition by a three compartment model in adult Indian male and female subjects.

The body composition of 10 adult Indian male and female subjects was investigated by a three compartment model, using measurements of Total Body Water (TBW) by deuterium dilution, and of body density by hydrodensitometry. The three compartment model yielded significantly different (P < 0.005) estimates of percent body fat of 15.9+/-3.8 and 19.7+/-4.2% and of the Fat Free Mass (FFM) of 41+/-3.3 kg and 33.9+/-4.1 kg in the male and female subjects respectively. The hydration of the FFM was 0.704+/-0.032 in the males and 0.719+/-0.024 in the females; this difference was not signifcant between groups. The density of the FFM, measured from estimates of percent body fat by the 3 compartment approach and of body density by hydrodensitometry, was 1.107+/-0.014 in the males and 1.101+/-0.001 in the females with no significant differences between the groups. This study demonstrates differences in body composition between BMI matched healthy adult male and female subjects. Although there are significant differences for % Fat and FFM between the sexes, there are no significant differences in the hydration fraction and the density of the FFM.

Total body water measurements by deuterium dilution in adult Indian males and females.

Total Body Water (TBW) was measured in a group of 20 healthy adult Indian men and 10 women by the deuterated water dilution technique and their body composition was determined by applying a hydration factor of 0.7194 for fat free mass (FFM). The TBW in the male subjects whose mean body weight was 49.8 +/- 6.7 kg, was 60.6 +/- 3.2% of body weight (range 55.8%-65.4%), from which a FFM of 41.9 +/- 6.1 kg (range 31.8 kg-51.3 kg) was obtained. Total body water in the group of 10 female subjects whose mean body weight was 42.7 +/- 4.9 kg, was 57.0 +/- 4.5% of body weight (range 52.5%-64.2%) from which a FFM of 34.0 +/- 5.1 kg (range 28.4 kg-39.4 kg) was obtained.